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Plant Regeneration through Somatic Embryogenesis in Common Bermudagrass Tissue Culture 1
Author(s) -
Ahn B. J.,
Huang F. H.,
King J. W.
Publication year - 1985
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1985.0011183x002500060049x
Subject(s) - biology , somatic embryogenesis , callus , cynodon dactylon , inflorescence , botany , sucrose , tissue culture , poaceae , horticulture , embryogenesis , embryo , biochemistry , in vitro , microbiology and biotechnology
Tissue culture techniques could be useful for improving common bermudagrass ( Cynodon dactylon (L.) Pers.). Calli originating from immature inflorescences grew larger on a modified N 6 than on MS medium. Both media contained 1 mg L −1 (2,4‐dicho lorophenoxy) acetic acid (2,4‐D) and either 20 or 60 g L −1 sucrose. Embryogenic calli were produced from 16 of 19 immature inflorescences, less than 0.5 cm long, on Ne medium with 1 mg L −1 2,4‐D and 60 g L −1 sucrose, while only non‐embryogenic calli were induced from those over 1.5 cm length. Embryogenic calli forming somatic embryos spontaneously have been maintained without loss of competence for 80 weeks by subculturing at 4‐week intervals. Numerous plantlets were regenerated from each callus upon transfer to hormone‐free Ne medium and were grown to maturity in soil. Scanning electron microscopy confirmed the bermudagrass regeneration through embryogenesis.