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Nodulation Mutants in Chickpea 1
Author(s) -
Davis Thomas M.,
Foster K. W.,
Phillips Donald A.
Publication year - 1985
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1985.0011183x002500020033x
Subject(s) - biology , inoculation , rhizobium , nitrogen fixation , horticulture , rhizobiaceae , mutant , strain (injury) , botany , symbiosis , gene , genetics , bacteria , anatomy
As a first step toward future studies of symbiotic N 2 fixation in chickpea ( Cicer arietinum L.), genotypes exhibiting aberrant responses to inoculation with Rhizobium strain CC1192 were isolated by selection following γ,‐irradiation mutagenesis of chickpea line P502. In greenhouse experiments, 28 of 968 M 2 families contained one or more plants which showed stunted, chlorotic growth after 30 to 35 days under N 2 ‐dependent conditions but which resumed vigorous growth with the addition of 8 m M NH 4 NO 5 . Of 24 selections advanced to the M 4 generation, two lines, PM233 and PM665, rep:roducibly failed to form root nodules under various conditions where the parental control P502 was well nodulated. PM233 and PM665 grew vigorously with 8 mM NH 4 NO 5 , but PM665 could be distinguished from the wild type by lighter green leaves and slightly crinkled pods. Six other lines exhibited aberrant responses to inoculation in replicated M 4 ‐generation testing, but have not yet been satisfactorily characterized.