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Long‐Term Preservation of Hop Pollen in Liquid Nitrogen 1
Author(s) -
Haunold Alfred,
Stanwood P.C.
Publication year - 1985
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1985.0011183x002500010047x
Subject(s) - pollen , biology , germination , pollination , germplasm , horticulture , botany , hop (telecommunications) , humulus lupulus , liquid nitrogen , pollen source , agronomy , chemistry , pollinator , computer network , organic chemistry , pepper , computer science
This research was initiated to evaluate long‐term hop (`Humulus lupulus`` L.) pollen storage for breeding purposes and germplasm preservation. Air‐dried pollen was stored in a refrigerator (3°C), a freezer (−18°C), and in liquid nitrogen (−196°C) and compared with fresh pollen by hand pollination of receptive female flowers and subsequent seed germination tests. Pollen stored in liquid N averaged four seeds per cone in test crosses over a 2‐year period as compared to slightly over five seeds per cone from fresh pollen. Pollen stored at −18°C lost about half of its viability after 1 year, while pollen stored at 3°C became nonfunctional within 1 year. Reducing moisture content of fresh pollen to about 10% prior to cold temperature storage was essential.