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Polar‐Glycerolipid Metabolism in Soybean Seed with Genetically Altered Unsaturated Fatty Acid Composition 1
Author(s) -
Carver Brett F.,
Wilson Richard F.
Publication year - 1984
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1984.0011183x002400060005x
Subject(s) - biology , linoleic acid , cultivar , genotype , linolenic acid , glycine , oleic acid , composition (language) , fatty acid , botany , zoology , food science , horticulture , biochemistry , amino acid , gene , linguistics , philosophy
Recurrent selection methodology has been utilized in the development of a soybean [ Glycine max (L.) Merr.] genotype, designated as N78‐2245, that contains a high concentration of oleic acid (18:1) and lower concentrations of linoleic (18:2) and linolenic (18:3) acids compared to seed from typical soybean cultivars. Ontogenetic changes in total polar‐glycerolipid (TPL) composition were characterized for the first time in N78‐2245 and in ‘Dare’, a typical soybean cultivar. The objective of this work was to determine whether the overall rate of 18:l‐desaturation changed with increased seed for a given genotype and to assess the level of 18:l‐desaturase activity in genotypes that differed in 18:1 content. Phosphatidylcholine (PC) was found to be more active metabolically than any other polar‐glycerolipid. Genotypic differences in the concentration of PC species enriched in 18:1 were expressed throughout seed growth. At 45 days after flowering the 18:1‐PC concentration in the high‐18:l genotype, N78‐2245, was 4.2‐times greater than that in the standard cultivar, Dare. As these genotypes matured the concentration of 18:1‐PC declined, and the concentration of species enriched with linoleic acid (18:2) increased. The overall rate of 18:1‐PC desaturation activity did not change with increased seed age in either genotype. Hence developmental changes in 18:1‐PC species concentration were not the result of changes in acyldesaturase activity. A reduced rate of overall desaturation activity in N78‐2245 as compared with Dare, however, may have contributed to the higher levels of 18:1 in glycerolipids from N78‐2245 seed.