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Confirmation by Mitochondrial DNA Analysis of a Euplasmic Spring Wheat Expressing a Dominant Male‐Sterility Gene 1
Author(s) -
Altman D. W.,
Storey K. K.,
Gengenbach B. G.
Publication year - 1984
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1984.0011183x002400030039x
Subject(s) - biology , sterility , cytoplasmic male sterility , mitochondrial dna , genetics , restriction enzyme , gene , allele , cytoplasm , microbiology and biotechnology
A dominant male‐sterility gene has had limited utilization in wheat ( Triticum aestivum L.) breeding partly because appropriate lines have alien ( T. tauschii Schmal.) cytoplasm. The dominant allele for male‐sterility was though to have been transferred into a euplasmic ( T. aestivum cytoplasm) background, but the cytoplasmic constitution of this line had not been confirmed. Electrophoretic gel patterns of mitochondrial DNA (mtDNA) a putative euplasmic line which segregates for the dominant male‐sterility allele were compared with gel patterns of mtDNA of known T. aestivum and T. tauschii cytoplasm lines following digestion with the restriction endonucleases Pst I, Eco Rl, Bam HI, Sal l, Xho l, and Hind lll. All restriction patterns of the putative euplasmic line verified that the cytoplasm was from T. aestivum . Euplasmic sources of dominant male‐sterility should have greater value than alloplasmic lines for future genetic and breeding studies using this gene.