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Locating the w 1 Locus on the Satellite Chromosome in Soybean 1
Author(s) -
Sadanaga K.,
Grindeland R. L.
Publication year - 1984
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1984.0011183x002400010034x
Subject(s) - biology , locus (genetics) , chromosomal translocation , genetics , chromosome , breakpoint , pollen , hybrid , microbiology and biotechnology , botany , gene
F 2 data of field‐grown plants of a cross between translocation 172‐11‐3 (purple flower, W 1 ) and type collection line T93A (white flower, w 1 ) of soybean [ Glycine max (L.) Merr.] revealed a significant deviation from the expected ratio of 3 purple: 1 white. To determine the cause(s) of the abnormal ratio, the chromosome number and constitution of the F 2 progenies of another cross involving 172‐11‐3 and T161 (white flower) were determined. Translocation 172‐11‐3 has cytologically recognizable interchange chromosomes, and the short interchange chromosome was present as a univalent in 46% of the pollen mother cells (PMCs) in interchange heterozygotes. Sixty‐five percent of the F 2 progeny were disomics of the N/N, N/T, and T/T types, 18% were trisomics, 16% were 40‐chromosome plants with a duplication‐deficiency gamete, and 1% were 42‐chromosome plants. Assuming that the univalent was lost in the cytoplasm 50% of the time, the observed number of N/N and N/T plants fit the expected, but the number of T/T did not. A recombination frequency of 1.9% between w 1 and the breakpoint was calculated. The F 2 data of duplex trisomic hybrids indicated that the w 1 locus was on the satellite chromosome. The breakpoint on the long arm of the satellite chromosome is distal to the w 1 locus. Our data indicated no linkage between the breakpoint and the genes y 10 (chlorophyll deficiency) and t (pubescence color).