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Differences in Endosperm Proteins Between Yellow Berry and Normal Triticales
Author(s) -
Bietz Jerold A.,
Sharma Govind C.
Publication year - 1983
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1983.0011183x002300040024x
Subject(s) - endosperm , glutenin , biology , size exclusion chromatography , triticale , biochemistry , poaceae , gel electrophoresis , botany , enzyme , gene , protein subunit
Endosperm proteins involved in the expression of the yellow berry (YB) phenomena in triticale ( X Triticosecale Wittmack) were investigated. The protein fractions isolated from single kernels of normal and YB triticales were characterized by electrophoresis combined with densitometry and by high‐performance gel filtration chromatography. Electrophoresis of gliadins revealed significant qualitative differences between YB and normal types; in addition, multiple biotypes were apparent in some lines. Reduced glutenin subunits of normal and YB triticales had very similar molecular weight distributions, but subtle differences in amino acid composition and sequence were revealed by different detection methods. Results indicated that normal triticales contain more residue protein (unreduced high molecular weight glutenin) than YB kernels. Consequently, numerous subtle quantitative and qualitative differences in amounts of various protein types seem to influence interactions of proteins with each other and with starch. These interactions may lead to the differences in endosperm vitreosity and hardness between normal and YB kernels.