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Long‐Term Pollen Storage of Pearl Millet 1
Author(s) -
Hanna W. W.,
Wells H. D.,
Burton G. W.,
Monson W. G.
Publication year - 1983
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1983.0011183x002300010050x
Subject(s) - pennisetum , pollen , biology , pearl , pollination , horticulture , moisture , botany , agronomy , chemistry , philosophy , theology , organic chemistry
The purpose of this research was to develop a practical method for long‐term pollen storage of pearl millet ( Pennisetum americanum L. Leeke). Pearl millet pollen with 7.5% moisture stored for 185 days in plastic zip‐lock bags at –73 C gave 100% seed set when used to pollinate cytoplasmic male sterile (cms) ‘Tift 23DA’ heads. Pollen dried to 2.2% also gave 100% seed set on cms heads after 129 days of storage. Frozen pollen with 6 to 7% moisture content that was thawed for 3 hours, refrozen, and later used in crosses produced 100% seed set.