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The Effect of Rhizobium trifolii Strains and Crimson Clover Genotypes on N2 Fixation 1
Author(s) -
Smith G. R.,
Knight W. E.,
Peterson H. L.,
Hagedorn C.
Publication year - 1982
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1982.0011183x002200050017x
Subject(s) - biology , rhizobium , randomized block design , dry weight , nitrogen fixation , strain (injury) , horticulture , agronomy , botany , inoculation , bacteria , genetics , anatomy
The effects of crimson clover ( Trifolium incarnatum L.) genotype and strain of Rhizobium trifolii on dry weight yield and N 2 fixation were investigated in a factorial experiment. The treatments were arranged in a randomized complete block design with eight replications. The clover‐ Rhizobium combinations were grown in plastic pouches with a N‐free nutrient solution. A growth chamber was used to maintain 24/19 C diurnal temperatures and a 12 hour photoperiod. After 60 days of growth, the combinations were assayed for N 2 fixation using acetylene reduction and the plant tops were harvested and dried. Plant lines, strains of Rhizobium and line ✕ strain interaction were all significant sources of variation for both acetylene reduction rate and dry weight yield. Strains had the largest effect, in terms of variance components, on dry weight yield followed by plant lines and strain ✕ line interaction, respectively. Strains also had the largest effect on acetylene reduction rate followed by strain ✕ line interaction and plant lines, respectively. Strain TA1 of R. trifolii was highly variable in its effect on the different plant lines. Response of different inbred lines with this strain ranged from efficient to inefficient.