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Variation among Inbred Lines of Crimson Clover for N 2 Fixation (C 2 H 2 ) Efficiency 1
Author(s) -
Smith G. R.,
Knight W. E.,
Peterson H. H.
Publication year - 1982
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1982.0011183x002200040005x
Subject(s) - acetylene , biology , nitrogen fixation , inoculation , rhizobium , coefficient of variation , horticulture , dry matter , inbred strain , botany , nitrogen , bacteria , chemistry , genetics , gene , chromatography , organic chemistry
Inbred lines of crimson clover ( Trifolium incarnatum L.) were assayed for N 2 fixation using the acetylene reduction assay. The seedlings were inoculated with a single pure strain (162K13) of Rhizobium trifolii and cultured in a nil‐nitrogen nutrient solution contained in individual plastic growth pouches. The acetylene reduction assay was carried out on live plants using the plastic pouches to contain the gas mixture. In the first experiment, significant differences were noted among 14 crimson clover lines for acetylene reduction. Top dry matter, leaf number, and visual scores of nodulation, root growth and top growth were significantly correlated with the acetylene reduction assay. A relatively large coefficient of variation (27.1%) was noted for this experiment. In the second experiment refinement of culture and assay techniques resulted in a lower coefficient of variation (15.2%) and significant differences among six crimson clover lines for acetylene reduction were found.