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Use of Hypodermic Inoculations of Alfalfa for Identifying Host Reactions and Races of Colletotrichum trifolii 1
Author(s) -
Ostazeski S. A.,
Elgin J. H.
Publication year - 1982
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1982.0011183x002200030026x
Subject(s) - inoculation , biology , hypodermic needle , conidium , horticulture , botany , syringe , psychology , psychiatry
A hypodermic inoculation technique was used to nondestructively determine the differing resistance to race 1 and race 2 of alfalfa plants in breeding and genetic studies. Stem inoculations were made with hypodermic syringes fitted with 23‐27 gauge needles. Conidial concentrations ranged from 1 to 9 million conidia/ml. Symptoms could be classified in 10 to 14 days. Susceptible stems formed large lesions at the inoculation site and wilted and collapsed. Resistant stems continued to grow normally with no lesion development. Hypodermic inoculations resulted in disease resistance reactions similar to those obtained with conventional spray inoculation techniques. We have isolated clones with the following combinations of resistant ( + ) and susceptible (−) reactions to race 1 and race 2, respectively: + +, + −, and − −.