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Enzyme‐Linked Immunosorbent Assay for the Determination of Lipoxygenase Isoenzymes in Soybean 1
Author(s) -
Yabuuchi Seizo,
Lister Richard M.,
Axelrod Bernard,
Wilcox James R.,
Nielsen Niels C.
Publication year - 1982
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1982.0011183x002200020032x
Subject(s) - lipoxygenase , biology , enzyme , isozyme , glycine , biochemistry , affinity chromatography , enzyme assay , microbiology and biotechnology , chromatography , amino acid , chemistry
An enzyme‐linked immunosorbent assay (ELISA) was developed for the quantitative determination of lipoxygenase‐1 (L 1 ) and lipoxygenase‐ 3 (L 3 ) in soybeans ( Glycine max (L.) Merr.). Monospecific goat ( Carpa hircus ) and rabbit ( Oryctolagus cuniculus ) immunoglobulins purified by affinity chromatography were used for the L 1 and L 3 assays, respectively. In both cases the coefficients of variation in the assays were about 10%. A linear relationship between lipoxygenase (L 1 ) enzymic activity and the enzyme protein content as determined by ELISA was obtained. The technique has considerable potential for screening of segregating soybean populations for genotypes deficient in specific lipoxygenase isozymes.