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Isoenzyme Polymorphism in St. Augustinegrass 1
Author(s) -
Green R. L.,
Dudeck A. E.,
Hannah L. C.,
Smith R. L.
Publication year - 1981
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1981.0011183x002100050037x
Subject(s) - isozyme , biology , acid phosphatase , biochemistry , polyacrylamide gel electrophoresis , alcohol dehydrogenase , microbiology and biotechnology , enzyme , phosphatase
St. Augustinegrasses ( Stenotaphrum secundatum Walt. Kuntze) were investigated to determine the feasibility of their identification by isoenzyme variability. Several Florida clones of this species are morphologically indistinguishable especially when grown as turfgrasses under high fertility. Adenosine diphosphate glucose pyrophosphorylase, UDP glucose pyrophosphorylase, alcohol dehydrogenase (ADH), and acid phosphatase isoenzymes extracted from leaf tissue of 28 clones were separated by polyacrylamide disc electrophoresis. Gels of all 28 clones were stained for ADP glucose pyrophosphorylase and UDP glucose pyrophosphorylase isoenzyme activity while gels from selected clones were stained for total protein, ADH, and acid phosphatase isoenzymes. Clones could be divided into several groups by quantitative differences of bands with equal electrophoretic mobility of ADP glucose and UDP glucose pyrophosphorylases. St. Augustine grasses grown in Florida could be divided into two groups by either ADP glucose or UDP glucose pyrophosphorylase isoenzyme systems or total protein. These groups could be further subdivided by acid phosphatase and ADH isoenzymes.