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Tissue Culture of Plants from a Chimeral Mutation of Tobacco 1
Author(s) -
Kasperbauer M. J.,
Sutton T. G.,
Andersen R. A.,
Gupton C. L.
Publication year - 1981
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1981.0011183x002100040028x
Subject(s) - biology , nicotiana tabacum , meristem , ploidy , botany , axillary bud , tissue culture , shoot , explant culture , somatic cell , sucker , protoplast , solanaceae , genetics , gene , in vitro , anatomy
Somatic mutations may contain genetic characteristics usable in plant improvement. Meristem tissue was cultured to obtain healthy plants from a chimeral mutation of a tobacco ( Nicotiana tabacum L.) plant that was infected with tobacco vein mottling virus. Parts of some of the leaves originating from a narrow mutated strip on one side of the stalk exhibited a ruffled characteristic. The other leaves on the plant were smooth. Thin layers of cells from the meristematic domes of apical and axillary buds were excised from an axillary shoot (sucker) that originated at the edge of the mutated strip. The explants were cultured on a shoot regeneration medium. The regenerated plants included two that had ruffled and 59 that had smooth leaves. Regenerated plants of both leaf types had the normal somatic chromosome number, did not exhibit virus symptoms, were self‐fertile, and produced abundant seed. Selfed progeny of the regenerated ruffled‐leaf plants included some with smooth and some with ruffled leaves; whereas, all progeny of the regenerated smooth‐leaved plants had smooth leaves. Selfed progeny of doubled haploids cultured from a ruffled‐leaf haploid plant had ruffled leaves, and selfed progeny of doubled haploids cultured from a smooth‐leaved haploid plant had smooth leaves. Recovery of somatic mutants using tissue culture offers a means of obtaining plants with potentially useful mutated characteristics.