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In Vitro Responses of Tolerant and Susceptible Lines of Lotus corniculatus L. to 2,4‐D 1
Author(s) -
Swanson E. B.,
Tomes D. T.
Publication year - 1980
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1980.0011183x002000060029x
Subject(s) - lotus corniculatus , callus , biology , trefoil , in vitro , lotus , botany , horticulture , biochemistry , agronomy
A positive relationship was established between the in vivo and in vitro 2,4‐D [(2,4‐dichIorophenoxy) acetic acid] response of two lines of birdsfoot trefoil ( Lotus corniculatus L.). Callus cultures were initiated from a line selected for 2,4‐D tolerance in the field (T‐68), and from a susceptible cultivar ‘Leo.’ The response of the two callus lines was compared with 2,4‐D levels lower than the maintenance medium (<1 mg/liter), on progressively higher levels (5, 10, 15, and 20 mg/liter), and on high levels (40 mg/liter) throughout seven subcultures. The Leo callus was more responsive to both decreased, and moderately increased levels of 2,4‐D. Growth of both callus cultures was severely inhibited at high levels of 2,4‐D. In vitro selection for 2,4‐D tolerance appears to be practicable in Lotus corniculatus L. Indications are that 2,4‐D conjugation is one possible mechanism for differential tolerance in this species.