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Comparison Between Kjeldahl and Near Infrared Protein Analyses on Vegetative and Head Samples of Wheat 1
Author(s) -
Klepper Lowell,
Wilhelmi Kenneth
Publication year - 1979
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1979.0011183x001900060027x
Subject(s) - kjeldahl method , biology , cultivar , autoanalyzer , chromosomal translocation , botany , agronomy , nitrogen , chromatography , chemistry , biochemistry , organic chemistry , gene
A Technicon Dickey‐john Near Infrared (NIR) Analyzer (lnfra Alyzer) was used to determine if NIR analyses could be used to study protein translocation in wheat ( Triticum aestivum L.). It was calibrated, using Kjeldahl protein values, for wheat head and vegetative protein. These calibrations were tested with samples from a field experiment involving different cultivars, different levels of N application, and time of application. Correlation coefficients (r) were calculated between Kjeldahl and NIR protein from head emergence to maturity. For wheat head protein, the overall r = +0.82 and for wheat vegetative protein, r =‐ +0.97. It was concluded that NIR analyses were sufficiently accurate to be utilized in wheat protein translocation studies.