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Tissue Culture of Annual Ryegrass ✕ Tall Fescue F 1 Hybrids: Callus Establishment and Plant Regeneration 1
Author(s) -
Kasperbauer M. J.,
Buckner R. C.,
Bush L. P.
Publication year - 1979
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1979.0011183x001900040008x
Subject(s) - biology , callus , festuca arundinacea , subculture (biology) , lolium multiflorum , peduncle (anatomy) , hybrid , botany , tissue culture , somaclonal variation , poaceae , micropropagation , explant culture , horticulture , agronomy , in vitro , biochemistry
Calluses were established from internode and peduncle explants of field‐grown annual ryegrass ( Lolium multiflorum Lam.) ✕ tall fescue ( Festuca arundinacea Schreb.) F 1 hybrids, and plants were regenerated from the calluses. Modified MS medium with different levels of 2,4‐dichlorophenoxyacetic acid (2,4‐D) was used to initiate (2 to 4 mg of 2,4‐D/liter) and maintain (2 mg/liter) callus and to regenerate (0.25 mg/liter) plants. Plants regenerated after the first two subcultures at 3 to 4‐week intervals appeared to be identical in appearance and chromosome number to the original source plants. The doubled chromosome number was obtained in some plants regenerated after an 8‐week subculture period in which the callus approached senescence. Increasing the number of subcultures before regeneration increased the frequency of phenotypic variants among the regenerants. The techniques described offer an opportunity for forage grass modification via cell and tissue culture.