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Regeneration of Plantlets from Callus Cultures of Indiangrass 1
Author(s) -
Chen C. H.,
Lo P. F.,
Ross J. G.
Publication year - 1979
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1979.0011183x001900010029x
Subject(s) - callus , kinetin , biology , organogenesis , murashige and skoog medium , botany , horticulture , tissue culture , in vitro , biochemistry , gene
Plantlets were regenerated through organogenesis from the callus cultures of 10 agronomically superior genotypes of Indiangrass ( Sorghastrum nutans L Nash). Callus was first initiated from segments of young inflorescences explanted on RM medium supplemented with hormonal combinations of 5 or 10 mg 2,4‐D and 0 or 0.2 mg kinetin/liter. Plantlets were then induced from the calluses by subculturing on RM medium with kinetin omitted and 2,4‐D reduced below 1 mg/liter. Of the media tested, callus grew best on RM medium supplemented with 5 mg 2,4‐D and 0.2 mg kinetin/liter. Differential responses to the morphogenetic induction medium were noted among the 10 genotypes tested.