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Haploid and Diploid Callus from Cotton Anthers 1
Author(s) -
Barrow Jerry,
Katterman Frank,
Williams Dale
Publication year - 1978
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1978.0011183x001800040024x
Subject(s) - callus , ploidy , stamen , biology , botany , tissue culture , gossypium barbadense , gossypium , gossypium hirsutum , genetics , pollen , gene , in vitro
We have initiated and subcultured large quantities of callus tissue from the anthers of two species of cotton, Gossypium barbadense L . and G. hirsutum L . A liquid medium was developed and cell suspension cultures were generated from anther‐derived callus from G. hirsutum . Cytological examination was made only on anther callus from G. hirsutum and the preponderance of the tissue was diploid (2n=4x=52). Liquid suspension cultures from anther callus were also found to be primarily diploid. However, 13 cultures that grew more than four times faster than 527 diploid cultures had 83% haploid cells. Therefore, haploid tissue was produced in 2 to 3% of the anthers, and it grew obscurely within the diploid callus. The haploid tissue was identified and selected by its rapid growth rate in liquid culture.