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A Colorimetric Screening Assay for Lysine in Wheat Using Trinitrobenzesulfonic Acid 1
Author(s) -
Felker Peter,
Labanauskas C. K.,
Waines Giles
Publication year - 1978
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1978.0011183x001800030034x
Subject(s) - lysine , biology , colorimetry , amino acid , amino acid analysis , chromatography , biochemistry , food science , chemistry
A colorimetric lysine screening assay in wheat is reported that employs trinitrobenzenesulfonic acid, which reacts only with primary amino proups. When lysine in 26 wheat samples is compared with that determined by traditional amino acid analysis, the assay has a correlation coefficient of 0.938. It is possible to perform 48 lysine determinations per man‐day.