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Use of 4‐Amino‐3,5,6‐trichloropicolinic Acid as an Auxin Source in Plant Tissue Cultures 1
Author(s) -
Colins G. B.,
Vian W. E.,
Phillips G. C.
Publication year - 1978
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1978.0011183x001800020023x
Subject(s) - picloram , auxin , biology , callus , nicotiana tabacum , canavalia ensiformis , 2,4 dichlorophenoxyacetic acid , glycine , tissue culture , botany , explant culture , solanaceae , dicamba , 1 naphthaleneacetic acid , amino acid , horticulture , biochemistry , in vitro , agronomy , cytokinin , weed control , gene
The auxin‐like properties of 4‐amino‐3,5,6‐trichloropicolinic acid (picloram) were compared with those of other auxins in tissue cultures of wheat ( Triticum aestivum ), soybeans ( Glycine max ), tobacco ( Nicotiana tabacum ), and jackbean ( Canavalia ensiformis ) explants. Certain problems associated with the use of 2,4‐dichlorophenoxyacetic acid (2,4‐D) in tissue culture were not encountered when picloram was substituted for 2,4‐D in the culture medium of 2,4‐D‐requiring plant materials. Picloram performed as well or better than the auxins to which it was compared when used in plant tissue cultures. In general, picloram was more effective at low concentrations than the other auxins. The data indicate potential uses of picloram in routine callus cultures, in regeneration of plants from calli, and in research concerning the physiological development of plant tissues.