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Clonal Propagation of Big Bluestem by Tissue Culture 1
Author(s) -
Chen C. H.,
Stenberg N. E.,
Ross J. G.
Publication year - 1977
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1977.0011183x001700060007x
Subject(s) - callus , biology , andropogon , kinetin , vermiculite , botany , tissue culture , murashige and skoog medium , inflorescence , sucrose , horticulture , food science , biochemistry , in vitro
Callus of big bluestem ( Andropogon gerardii Vitman) was induced from 5 to 10 mm segments of young inflorescences cultured on Linsmaier and Skoog (RM) solid medium supplemented with 5 mg of 2,4‐dichlorophenoxyacetic acid (2,4‐D) and 0.2 mg kinetin/liter in the dark at 25 C. The callus condition was maintained by subculturing on RM medium supplemented with 5 mg of 2,4‐D/liter. Differentiation of numerous plants from callus occurred on the same basal medium with the 2,4‐D concentration reduced below 2 mg/liter. The plants developed normally if grown under light on RM medium free of hormones. At the two‐leaf stage, the plantlets were transplanted into vermiculite and subsequently to the field or into greenhouse pots with a survival rate as high as 85%.