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Root and Shoot Development from Sugarcane Callus Tissue 1
Author(s) -
Nadar H. M.,
Heinz D. J.
Publication year - 1977
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1977.0011183x001700050035x
Subject(s) - shoot , biology , callus , saccharum , botany , tissue culture , horticulture , murashige and skoog medium , in vitro , biochemistry
Growth of shoots differentiated from sugarcane ( Saccharum sp.) callus was enhanced by 5 mg naphthalene acetic acid (NAA)/I in the absence of coconut water, while 0.2 mg 2,4‐dichlorophenoxyacetic acid (2,4‐D)/1 stunted their growth. In the presence of NAA, a crown developed at the base of the shoot on which new shoots and roots developed, grew, and formed a clump of plantlets. Root growth could also be induced by trimming the leaves or by incubation at 15C for 4 to 6 weeks. Leaf trimming resulted in more vigorous root growth than cold treatment, although clonal differences were observed. Results indicate that a medium which stimulates differentiation does not necessarily stimulate shoot growth, and the hormonal requirements for shoot differentiation differ from requirements for root induction in sugarcane callus.