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Inheritance of a Second Trypsin Inhibitor Variant in Seed Protein of Soybeans 1
Author(s) -
Orf J. H.,
Hymowitz T.
Publication year - 1977
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1977.0011183x001700050034x
Subject(s) - biology , trypsin inhibitor , locus (genetics) , glycine , botany , kunitz sti protease inhibitor , allele , horticulture , trypsin , genetics , gene , biochemistry , amino acid , enzyme
Soybean [ Glycine max (L.) Merrill] seeds from selfed heterozygous F 2 plants of the cross ‘Harosoy’ ✕ P‐I. 246367 and F 2 seeds from reciprocal crosses of T245 and P.I. 246367 were analyzed for electrophoretic banding patterns of SBTI‐A 2 (Kunitz inhibitor). Harosoy seed has the SBTI‐A 2 band at Rf 0.79 ( Ti 1 ), T245 at Rf 0.75 (Ti 2 ) and P.I. 246367 at Rf 0.83. Seeds having two bands produced plants in which seeds segregated in a 1:2:1 ratio whether in the F 2 , F 3 , or F 4 generation. All seed with only one band bred true in the next generation. These data along with data previously reported establishes that three forms of the SBTI‐A 2 designated as Ti 1 , Ti 2 , and Ti 3 are electrophoretically distinguishable from one another by their different Rf values of 0.79, 0.75, and 0.83, respectively. The forms are controlled by a codominant multiple allelic system at a single locus