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Relationship of Ribonucleic Acid Degrading Enzymes to Survival Levels of Winter Barley Phenotypes 1
Author(s) -
Kenefick D. G.,
Kolp B. J.,
Whitehead E. I.
Publication year - 1977
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1977.0011183x001700050018x
Subject(s) - ribonuclease , biology , sterility , enzyme , hordeum vulgare , phosphodiesterase , phenotype , hordeum , enzyme assay , rnase p , biochemistry , poaceae , genetics , botany , rna , gene
The ribonuclease and phosphodiesterase enzyme activities of 18 lines of barley ( Hordeum vulgare L.) were compared to their winter survival ratings. These lines, selected in Wyoming, were obtained from a composite cross derived from 18 original parents using male sterility. Enzyme analysis on the soluble extract from 4‐day seedlings showed, in general, that the rihonuclease activity in the hardiest phenotypes was depressed to a greater extent by 0.50 M KCI. These extracts contained the lowest amounts of an enzyme tentatively identified as ribonuclease L Usually the extracts of the hardiest phenotypes also contained the highest phosphodiesterase activity, as determined with thymidine 3′‐(p‐nitrophenyl) phosphate.