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Ethidium Bromide Induced Cytoplasmic Male Sterility in Pearl Millet 1
Author(s) -
Burton Glenn W.,
Hanna Wayne W.
Publication year - 1976
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1976.0011183x001600050035x
Subject(s) - pennisetum , ethidium bromide , biology , software maintainer , mutant , sterile water , sterility , cytoplasmic male sterility , horticulture , botany , genetics , gene , dna
Seeds of homozygous ‘Tift 23DB 1 ’ pearl millet [ Pennisetum americanum (L.) K. Schum], maintainer for cytoplasmic male sterile (cms) ‘Tift 23DA 1 ,’ were soaked in deionized water solutions of 250 and 1,000 ppm of ethidium bromide (EB) at 5 C for 40 hours. Treated seeds were rinsed in tap water for 30 min, surface‐dried, and planted in replicated two‐row plots in the field with two‐row plots of untreated Tift 23DB 1 . Male‐sterile mutants (whole heads, sectored heads, or heads with intermingled male‐sterile and male‐fertile florets) occurred at frequencies of 1 in 30,100, I in 515, and 1 in 274 in total head populations of 30,100, 22,700, and 19,200 treated with 0, 250, and 1,000 ppm EB, respectively. Occurrence of M 1 sterile mutants that set seed when pollinated and gave rise to some cms progenies in the greenhouse proved that the mutants were cytoplasmic male sterile.