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Separation of Kentucky Bluegrass Cultivars using Peroxidase Isoenzyme Banding Patterns 1
Author(s) -
Wehner D. J.,
Duich J. M.,
Watschke T. L.
Publication year - 1976
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1976.0011183x001600040008x
Subject(s) - poa pratensis , seedling , cultivar , biology , peroxidase , horticulture , botany , agronomy , poaceae , enzyme , biochemistry
The peroxidase isoenzyme banding pattern of 15 Kentucky bluegrass ( Poa pratensis L.) cultivars was investigated for use in identification. Isoenzymes were separated by polyacrylamide slab gel electrophoresis, stained with benzidine‐H 2 0 2 , and the relative intensities of the bands evaluated with a densitometer. Cultivar separations were made on single seedling samples at the 1% level of confidence using band number, mobility, and intensity ratios. Overall variability of the procedure was evaluated using a horeradish peroxidase standard and the effects of seedling age and seedlot determined. Of the 15 cultivars, 11 could be separated individually, whereas the remaining 4 were grouped into two pairs. Band mobilities for the standard and the cultivars varied 1 to 3%, whereas intensity ratios varied 9% for the standard, and 5 to 20% for most cultivars. Neither seedlot, seedling age, nor leaf position on the same seedling affected the peroxidase isoenzyme banding pattern.