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Anther Culture of Triticale 1
Author(s) -
Ono H.,
Larter E. N.
Publication year - 1976
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1976.0011183x001600010033x
Subject(s) - triticale , kinetin , biology , callus , sucrose , stamen , botany , population , acetic acid , murashige and skoog medium , in vitro , ploidy , horticulture , tissue culture , pollen , food science , biochemistry , demography , sociology , gene
Anthers from three strains of triticale (X Triticosecale Wittmack) were cultured on B5 and Blaydes' basic media containing various concentrations and combinations of sucrose, 2,4‐D indole‐3‐acetic acid (IAA), kinetin, amino acids, and coconut milk. The three strains of triticale reacted differentially in their development of callus in vitro although the percentages were low for each. The highest response (4.0%) was obtained with anthers of ‘Rosner’ cultured on B5 medium with 10% sucrose and supplemented with 2 rag/liter of IAA, kinetin, and 2,4‐D. Of a total of 9,246 anthers cultured from the three strains of triticale, 80 calluses were transplanted to differentiating medium. From this population, seven haploid (n = 21) albino plantlets developed.