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Laboratory Technique for Bleaching Pea Seeds 1
Author(s) -
Riehle W. G.,
Muelbauer F. J.
Publication year - 1975
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1975.0011183x001500060031x
Subject(s) - sativum , petri dish , biology , pisum , germination , bleach , horticulture , filter paper , botany , chemistry , chromatography , organic chemistry , genetics
Conditions for bleaching pea ( Pisum sativum L.) seeds in the laboratory were studied to find a method of identifying bleach‐resistant selections in the early stages of a breeding program. Among the genotypes used, differential seed bleaching without germination was obtained when 4.2 g pea seed (about 25 seeds) was placed on five sheets of Whatman no. 1 filter paper in 9‐cm petri dishes and moistened with 6 ml water. The petri dishes were then placed in a growth chamber at 23 C with 8.6 klux of continuous light. After 8 days, the seed coats were removed, and the cotyledons were scored on a scale from 0 for no bleaching to 4 for complete bleaching. The technique was highly correlated (r = 0.78**, df = 27) with seed bleaching occurring in the field and should therefore be useful for early generation testing of green, dry‐edible pea selections.