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Influence of Osmotic Potential on the Growth and Development of Soybean Tissue Cultures 1
Author(s) -
Kimball S. L.,
Beversdorf W. D.,
Bingham E. T.
Publication year - 1975
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1975.0011183x001500060003x
Subject(s) - callus , sorbitol , mannitol , osmotic pressure , sucrose , glycine , biology , osmotic concentration , botany , osmotic shock , biophysics , tissue culture , biochemistry , turgor pressure , horticulture , food science , in vitro , amino acid , gene
The influence of reduced osmotic potentials on soybean ( Glycine max L.) tissue cultures was evaluated. Reduced osmotic potentials were generated by addition of mannitol, sorbitol, glucose, or sucrose to a standard medium. Cells in control callus were comparatively large and about half were elongated or irregularly shaped. As osmotic potential decreased, all cells became smaller and spherical, regardless of which osmoticum was used. The addition of metabolically active or inert osmotica resulted in increased callus production. Maximum callus growth (fresh weight) occurred when the osmotic potential of the culture medium was lowered 8 to 12 bars. Decreasing osmotic potentials by 2 to 20 bars did not increase the frequency of adventitious bud differentiation above the low frequency observed on the standard medium.