Premium
Physiological Significance of the In Vivo Assay for Nitrate Reductase in Cotton Seedlings 1
Author(s) -
Radin J. W.,
Sell C. R.,
Jordan W. R.
Publication year - 1975
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1975.0011183x001500050029x
Subject(s) - nitrate reductase , nitrate , in vivo , nitrogen assimilation , nitrite , biology , biochemistry , anaerobic exercise , assimilation (phonology) , food science , botany , microbiology and biotechnology , ecology , physiology , linguistics , philosophy
In vivo assay techniques for nitrate reductase activity were evaluated for their ability to predict nitrate assimilation rates of germinating dark‐grown cotton ( Gossypium hirsutum L.) seedlings. Activity was measured with four in vivo assay techniques: either aerobic or anaerobic, and either with or without added nitrate in the assay medium. Assays without added nitrate in the medium allowed closer estimates of reduced N accumulation than did assays with added nitrate. Additionally, in the absence of added nitrate, the anaerobic assay provided a better estimate than the aerobic assay because of O 2 ‐stimulated nitrite reduction. The results suggest that the in vivo assay for nitrate reductase best approximated nitrate assimilation rates with only endogenous nitrate as substrate.