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Anther Culture in Soybeans 1
Author(s) -
Ivers D. R.,
Palmer R. G.,
Fehr W. R.
Publication year - 1974
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1974.0011183x001400060035x
Subject(s) - plantlet , biology , microspore , callus , stamen , kinetin , botany , cultivar , tissue culture , ploidy , starch , horticulture , greenhouse , pollen , biochemistry , gene , in vitro
To facilitate anther culture in soybeans [ Giycine max (L.) Merr.], five bud stages from the cultivar ‘Hark’ are described. Combinations of bud length, corolla development, and anther development were indicators used to classify buds as stages 1 through 5, Stage 3 contained microspores that were predominantly individualized, uninucleate, and devoid of starch. Culture contamination, which ranged from 3.3% with buds from the winter greenhouse, to 100% with material from the field, was best controlled by treatment with 95% ethanol for 2 min, 5.25%N aOCfIo r 5 min followed by a sterile water rinse. Miller's and Nitsch's basic media are evaluated for callus growth and plantlet development, with addition of various levels of coconut milk, kinetin, iron, and auxins. No embryoids or plantlets were observed and. the callus formed was diploid.