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Mitochondrial DNA from Maize Hybrids with Normal and Texas Cytoplasms 1
Author(s) -
Shah D. M.,
Levings C. S.
Publication year - 1974
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1974.0011183x001400060023x
Subject(s) - mitochondrial dna , biology , cytoplasmic male sterility , cytoplasm , cytosine , hybrid , extranuclear inheritance , dna , guanine , genetics , microbiology and biotechnology , botany , gene , nucleotide
Mitochondrial DNA (mtDNA) was isolated from two hybrids of maize ( Zea mays L.), NC232 ✕ T204N (normal cytoplasm) and NC232 ✕ T204 cms T (Texas cytoplasm). No difference was found in the buoyant densities of mtDNA isolated from the two hybrids. The mtDNA had a buoyant density of 1.706 g/cm 3 in CsCl and a molar % guanine plus cytosine of 46.9. Therefore, the cytoplasmic dissimilarities with respect to sterility and disease response between the normal and the Texas cytoplasm cannot be accounted for by detectable alterations in the base composition of mtDNA.