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Improved Technique for Evaluating Resistance of Corn to Corynebacterium nebraskense 1
Author(s) -
Calub A. G.,
Schuster M. L.,
Compton W. A.,
Gardner C. O.
Publication year - 1974
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1974.0011183x001400050032x
Subject(s) - inoculation , biology , horticulture , seedling , natural rubber , zea mays , agronomy , botany , chemistry , organic chemistry
An inoculating instrument consisting of multiple needles imbedded in a cork and foam rubber was effective in evaluating corn ( Zea mays L.) plants for reaction to leaf freckles and wilt disease caused by Corynebacterium nebraskense Schuster et al. Inoculum concentrations of about l0 8 bacterial cells/ml in the foam rubber resulted in infection and excellent differentiation between resistant and susceptible lines in both the greenhouse and field, 5 to 7 days after inoculation. Seedlings were pricked by the instruments, one in each hand, from the base of the whorl to the tip of the leaves. The correlation between ratings obtained with the pin‐prick method in the seedling stage and a syringe injection method at tasseling time was 0.977.