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Interaction of Shrunken ‐2 with Five Other Carbohydrate Genes in Corn Endosperm 1
Author(s) -
Holder D. G.,
Glover D. V.,
Shan J. C.
Publication year - 1974
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1974.0011183x001400050010x
Subject(s) - amylose , amylopectin , endosperm , starch , sucrose , carbohydrate , biology , polysaccharide , food science , fructose , botany , horticulture , biochemistry
The interactions of the shrunken ‐2 ( sh 2 ) gene with waxy ( wx ), amylose‐extender ( ae ), sugary ‐1 ( su 1 ), dull ( du ) and sugary ‐2 ( su 2 ) on the carbohydrate composition of maize ( Zea mays L.) endosperm were determined. The contents of fructose, glucose, sucrose, water‐soluble polysaccharide (WSP), and starch of whole kernel samples 21 and 28 days after pollination were determined. Percent starch and amylose of mature kernels were determined. The sh 2 mutation reduced starch drastically concurrent with a large accumulation of sucrose as previously observed. In homozygous double and triple recessive genotypes, sh 2 was epistatic over all other genes for the accumulation of sucrose and reduction of starch. Genotype combinations containing both su 1 and sh 2 produced the most extreme reduction in starch content, and in all cases sh 2 inhibited the large WSP content normally caused by su 1 . Starch from kernels homozygous for ae alone had 60% amylose. Presence of sh 2 in the multiple recessive genotypes inhibited the ae enhancement in percent amylose. With few exceptions those genotypes with wx produced no amylose; those which did were in combination with ae . Genotype combinations containing both wx and sh 2 , had higher sucrose than normal and the starch, although reduced in quantity, was 100% amylopectin.