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Sterile Culture of Immature Maize Seeds and Embryos 1
Author(s) -
Mock J. J.,
Dahmen W. J.
Publication year - 1973
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1973.0011183x001300060057x
Subject(s) - biology , germination , embryo , agar , sucrose , zea mays , botany , coat , embryo culture , pollination , poaceae , horticulture , agronomy , embryogenesis , bacteria , food science , ecology , pollen , genetics , microbiology and biotechnology
We cultured immature maize ( Zea mays L.) seeds and excised embryos (sampled at 5‐day intervals from 5 through 30 days after pollination) on solid‐agar nutrient medium containing 2% sucrose. Immature, intact seeds germinated poorly under our sterile culture conditions. Embryos germinated within seeds, but were incapable of emerging from the seed coat (even at 30 days postpollination). Excised embryos 15 days old and older germinated and developed normally.