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Effects of Genetic Background on Monogenic Resistance to Helminthosporium turcicum in Maize ( Zea mays L.) 1
Author(s) -
Calub A. G.,
Dunn G. M.,
Routley D. G.
Publication year - 1973
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1973.0011183x001300050020x
Subject(s) - biology , spore , phytoalexin , inoculation , inbred strain , germination , seedling , zea mays , poaceae , fungi imperfecti , horticulture , botany , agronomy , gene , genetics , biochemistry , resveratrol
A “drop‐diffusion” technique was used to determine the effects of genetic background upon the production of phytoalexin or other inhibitory substances in corn ( Zea mays L.) with monogenic resistance ( Ht/Ht ) to Helminthosporium turcicum Pass. Seedling leaves were inoculated with drops of an aqueous suspension of spores of H. turcicum in a humid chamber at 21C, 100% RH, and a light intensity of 3230 lux for up to 6 days. Drops of the suspension were then withdrawn from the leaf surfaces 1 to 6 days after inoculation and centrifuged. The supernatant or diffusate, then free of spores and germ tubes, was mixed with a drop of fresh spore suspension. Diffusates that inhibited spore germination or delayed the growth of the germ tube was assumed to contain phytoalexin or other inhibitory substance. Five standard susceptible inbred lines ( ht/ht ), their nearly isogenic resistant counterparts ( Ht/Ht ), and the five F 1 hybrids ( Ht/ht ) of paired isogenic lines were bioassayed. The incorporation of the Ht gene into any of the five inbred lines significantly increased the amount of inhibitory substances. Homozygous resistant inbred lines ( Ht/Ht ) produced significantly more phytoalexin or other inhibitory substances than the heterozygotes ( Ht/ht ) which in turn produced significantly more inhibition than susceptible inbred lines ( ht/ht ). The genetic background affected the amount and to a certain extent the time of production of inhibitory compounds. Bioassay tests and percent leaf infection following standard inoculation were not significantly correlated.