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A Root Tip Squash Technique for Soybean Chromosomes 1
Author(s) -
Palmer R. G.,
Heer Hollys
Publication year - 1973
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1973.0011183x001300030031x
Subject(s) - squash , biology , glycine , root tip , pectinase , horticulture , germination , botany , pellet , fixation (population genetics) , biochemistry , enzyme , gene , amino acid , zoology
A procedure is described for consistently obtaining adequatenumbers of well‐spread metaphase cells from soybean [ Glycine max (L.) Merr.] root tips. The salient features are: (i) seeds germinated at alternating temperatures of 30 C for 18 hours and 22 C for 6 hours until roots are 7 to 10 cm long; ii) pretreatment of root tips in saturated paradichlorobenzene at 15 C for 1½ to 2 hours; and iii) fixation in 3:1 for 48 hours at 37 C before hydrolysis and staining in leuco‐basic fuchsin. Tips were incubated in pectinase for 1½ hours at 30 C, and the cells were flattened with a pellet press.