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Identification of Hybrids from Alfalfa Crosses by Electrophoresis of Single Seed Proteins 1
Author(s) -
Miller M. K.,
Schonhorst M. H.,
McDaniel R. G.
Publication year - 1972
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1972.0011183x001200040047x
Subject(s) - biology , hybrid , medicago sativa , electrophoresis , botany , extraction (chemistry) , cultivar , genetics , chromatography , chemistry
A simple procedure far distinguishing hybrids from selfed alfalfas ( Medicago spp.) by extraction and separation of proteins from single alfalfa seeds using disc‐gel electrophoresis is described. One protein band was consistently light in three S 1 (first generation selfed) lines, consistently dark in another S 1 line; and varied in two other S 1 lines. Hybrids of parents with light and dark protein bands contained the protein at an intermediate concentration. Hybrids could thus be differentiated from selfs and crossing percentages determined. Variation in number and density of protein bands among plants within cultivars tested was very pronounced. Better extraction resulting in enrichment of bands was obtained when seeds were crushed rather than cut in half. Embryos and whole seeds produced similar bands.