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Germination of Cotton ( Gossypium hirsutum L.) Pollen on an Artificial Medium 1
Author(s) -
Taylor Richard M.
Publication year - 1972
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1972.0011183x001200020030x
Subject(s) - germination , pollen , pollen tube , biology , boric acid , petri dish , gossypium hirsutum , malvaceae , relative humidity , agar , botany , sucrose , horticulture , fiber crop , calcium nitrate , calcium , materials science , food science , chemistry , pollination , organic chemistry , genetics , physics , bacteria , metallurgy , thermodynamics
Gossypium hirsutum L. pollen was germinated by dusting it on a 3.5% agar medium containing sucrose, manganous sulfate, calcium nitrate, and boric acid. The medium was autoclaved for 5 min at 15 pounds per square inch and then poured into petri dishes to a depth of 5 to 7 mm. After cooling, the agar plates were stored in a refrigerator for 24 hours before use. Pollen was dusted onto the medium and the plates were placed in a germination chamber at 30 C and a relative humidity approaching 100%. To date, germinations have been low, ranging from 10 to 64% with an average of 30%. Advantages of this technique over the Broncker's method are: (i) more rapid germination (2 to 3 hours); (ii) more normal‐appearing tube cytoplasm, and (iii) much longer tubes.