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Colchicine‐Induced Polyploids from Cell Suspension Cultures of Sugarcane 1
Author(s) -
Heinz Don J,
Mee Grace W. P.
Publication year - 1970
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1970.0011183x001000060030x
Subject(s) - colchicine , polyploid , biology , callus , saccharum , saccharum officinarum , suspension culture , hybrid , botany , chromosome , chromosome number , horticulture , ploidy , cell culture , karyotype , genetics , gene
A high percentage of endoreduplicated chromosomes resulted when cell suspension cultures of sugarcane ( Saccharum officinarum L.) were treated with colchicine. Cytological examination of 46 plants from H 57‐1627 callus differentiated from the colchicine‐treated cells showed 22 to be polyploids, 2 to be mixoploids, and 22 to be diploids. This is the first report of chromosome doubling in commercial sugarcane hybrids. The proposed method of treating cells in suspension with colchicine should prove beneficial to sugarcane breeders and other breeders interested in obtaining polyploid plants.