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Uterotropic Activity and Heritability of Biochanin A and Formononetin in Trifolium medium L. 1
Author(s) -
Gourley L. M.,
Keim W. F.,
Stob Martin
Publication year - 1970
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1970.0011183x001000050015x
Subject(s) - biochanin a , formononetin , daidzein , genistein , isoflavones , red clover , biology , botany , biochemistry , genetics
The four isoflavones, genistein, biochanin A, formononetin, and daidzein, were identified in zigzag clover, Trifolium medium L. Biochanin A and formononetin were the predominant isoflavones; genisteln and daidzein were present only in trace amounts. The isoflavones were separated by thin‐layer chromatography and quantified spectrophotometrically. Biochanin A and formononetin concentrations were established for samples collected from two greenhouse and two field harvests of a complete six‐clone diallel cross. Analysis of variance by Griffing's combining‐ability procedure indicated that the significant genetic variance for the isoflavones was primarily explained by general combining ability. The upper leaflets of zigzag clover contained about 1/3 more biochanin A and formononetin than the lower ones, while the internodes contained about 1/10 as much formononetin as the leaflets, and only a trace of biochanin A.