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Two Rapid Assays for Saponin in Individual Alfalfa Plants 1
Author(s) -
Jones Merlyn,
Elliott Fred C.
Publication year - 1969
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1969.0011183x000900060002x
Subject(s) - saponin , biology , medicago sativa , fish <actinopterygii> , medicago , botany , saliva , biochemistry , medicine , alternative medicine , pathology , fishery , gene
Two assays were developed for screening individual alfalfa ( Medicago saliva ) plants for crude saponin. Twenty alfalfa leaf samples, selected on the basis of erythrocyte assay results, were evaluated by the fish assay and secondly by a chemical determination for saponins. The correlation coefficients of these assay results were ∝erythrocyte vs. chemical (r = .90), √fish vs. √erythrocyte (r = −.91), and √fish vs. chemical (r = −.81). Results indicate that the two assays may provide rapid and accurate estimates of crude saponin in alfalfa plants.