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Plant Differentiation from Callus Tissue of Saccharum Species 1
Author(s) -
Heinz Don J,
Mee Grace W. P.
Publication year - 1969
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1969.0011183x000900030030x
Subject(s) - callus , biology , saccharum , parenchyma , inflorescence , botany , tissue culture , shoot , clone (java method) , in vitro , biochemistry , gene
Callus tissue was induced on parenchyma tissue of shoot apices, leaves, and inflorescences of Saccharum sp. using a basal mineral medium supplemented with coconut water (10% by volume) and 2,4‐D. Transfer of callus tissue to a non‐2,4‐D basal medium induced the differentiation of plants. In all the plants studied, morphological variants from the parental clone were observed.