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Isocitric Dehydrogenase and Isocitric Lyase in Heterotic and Nonheterotic Maize ( Zea mays L.) 1
Author(s) -
Roos Eric E.,
Sarkissian Igor V.
Publication year - 1968
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1968.0011183x000800060013x
Subject(s) - isocitrate lyase , heterosis , hybrid , biology , lyase , isocitrate dehydrogenase , enzyme , imbibition , glyoxylate cycle , biochemistry , zea mays , botany , agronomy , germination
Heterotic and nonheterotic maize ( Zea Mays L.) hybrids and their inbred parents were studied during eariy postgermination to learn a physiological aspect of the phenomenon of heterosis. The enzymes isocltric dehydrogenase (L s ‐isocitrate:NADP oxidoreductase ldecarboxylating] EC 1.1.1.42) and isocitric lyase (L s ‐isocitrate glyoxylate‐lyase EC 4.1.3.1.) from scutellar tissue were assayed spectrophotometrically. When each hybrid was compared with its parents, none showed superior isocitric dehydrogenase activity during the period 2 to 6days following water imbibition. With respect to isocitric lyase activity, a definite heterotic response was observed in those hybrids classified as heterofic; nonheterotic hybrids failed to show heterosis for this enzyme. Lyase activity, first detectable 24 to 48 hours after imbibition, increased rapidly until day 5 and then declined. Differences in lyase activity appeared to be quantitative.