Localization of Anthocyanins by Peeling the Investments of the Barley Caryopsis 1

Three tissue layers may be peeled manually and successively from the caryopsis of barley at the dough stage, and at the flinty stage if first soaked in water at near freezing for 4 days. Correspondence of the peelable tissues to those named by histologists several decades ago were determined by sectioning with a cryostat. The technique has made possible a definitive chemical study of the anthocyanins and anthocyanogens of barley and precise grain color classifications of lines and varieties.