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A Comparison of Three Current Methods for the Analysis of Phytin Phosphorus 1
Author(s) -
Marrese R. J.,
Duell R. W.,
Sprague M. A.
Publication year - 1961
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1961.0011183x000100010023x
Subject(s) - library science , citation , state (computer science) , computer science , world wide web , algorithm
PHYTIN is an organic phosphate, defined by most investigators as the mixed calcium and magnesium salt of inositol hexaphosphoric acid (phytic acid). Half or more of the phosphorus of many mature seeds is reported as phytin (5). Phytin, then, is an important constituent of the total phosphorus in seeds and is of interest to many animal nutritionists. Reports of phytin investigations are clouded with inconsistencies, conflicting data, and differing theories (4). No firm agreement has been reached with respect to the complete structural formula of phytin. Methods of determination are indirect and involve many widely different procedures (1, 2, 5). Recent chromatographic investigations have shown the existence of the lower inositol phosphates in plants (1). These inositol mono-, di-, tri-, tetra-, and pentaphosphates probably react chemically much like phytin, the hexaphosphate, but, according to the prevailing definition, can not be considered as phytin. Some methods of analysis measure these lower inositol phosphates with the phytin, while others do not. One of the objectives of this investigation was to compare three current methods of phytin analysis. The following report presents some analytical data on this comparison.