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Fecal Indicator Bacteria and Antibiotic Resistance Genes in Storm Runoff from Dairy Manure and Compost‐Amended Vegetable Plots
Author(s) -
Jacobs Kyle,
Wind Lauren,
Krometis LeighAnne,
Hession W. Cully,
Pruden Amy
Publication year - 2019
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq2018.12.0441
Subject(s) - compost , manure , surface runoff , indicator bacteria , environmental science , agronomy , fertilizer , soil conditioner , fecal coliform , zoology , veterinary medicine , biology , soil water , water quality , ecology , medicine , soil science
Given the presence of antibiotics and resistant bacteria in livestock manures, it is important to identify the key pathways by which land‐applied manure‐derived soil amendments potentially spread resistance. The goal of this field‐scale study was to identify the effects of different types of soil amendments (raw manure from cows treated with cephapirin and pirlimycin, compost from antibiotic‐treated or antibiotic‐free cows, or chemical fertilizer only) and crop type (lettuce [ Lactuca sativa L.] or radish [ Raphanus sativus L.]) on the transport of two antibiotic resistance genes (ARGs; sul 1 and ermB ) via storm runoff from six naturally occurring storms. Concurrent quantification of sediment and fecal indicator bacteria (FIB; Escherichia coli and enterococci) in runoff permitted comparison to traditional agricultural water quality targets that may be driving factors of ARG presence. Storm characteristics (total rainfall volume, storm duration, etc.) significantly influenced FIB concentration (two‐way ANOVA, p < 0.05), although both effects from individual storm events (Kruskal–Wallis, p < 0.05) and vegetative cover influenced sediment levels. Composted and raw manure‐amended plots both yielded significantly higher sul 1 and erm B levels in runoff for early storms, at least 8 wk following initial planting, relative to fertilizer‐only or unamended barren plots. There was no significant difference between sul 1 or erm B levels in runoff from plots treated with compost derived from antibiotic‐treated versus antibiotic‐free dairy cattle. Our findings indicate that agricultural fields receiving manure‐derived amendments release higher quantities of these two “indicator” ARGs in runoff, particularly during the early stages of the growing season, and that composting did not reduce effects of ARG loading in runoff. Core Ideas Compost and manure soil amendments elevated FIB and ARGs in storm runoff. Composting manure prior to soil amendment did not reduce ARGs in runoff. Antibiotic and antibiotic‐free composts had similar quantities of ARGs and FIB. Vegetable type influenced sediment concentrations, but not FIB or ARGs, in runoff. Storm characteristics affected both FIB and ARG levels.