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Performance of Agricultural Residue Media in Laboratory Denitrifying Bioreactors at Low Temperatures
Author(s) -
Feyereisen Gary W.,
Moorman Thomas B.,
Christianson Laura E.,
Venterea Rodney T.,
Coulter Jeffrey A.,
Tschirner Ulrike W.
Publication year - 2016
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq2015.07.0407
Subject(s) - denitrifying bacteria , corn stover , nitrate , stover , straw , nitrous oxide reductase , chemistry , nitrous oxide , hordeum vulgare , bioreactor , crop residue , woodchips , denitrification , zoology , nitrogen , agronomy , environmental chemistry , poaceae , crop , food science , biology , ecology , agriculture , inorganic chemistry , organic chemistry , fermentation
Denitrifying bioreactors can be effective for removing nitrate from agricultural tile drainage; however, questions about cold springtime performance persist. The objective of this study was to improve the nitrate removal rate (NRR) of denitrifying bioreactors at warm and cold temperatures using agriculturally derived media rather than wood chips (WC). Corn ( Zea mays L.) cobs (CC), corn stover (CS), barley ( Hordeum vulgare L.) straw (BS), WC, and CC followed by a compartment of WC (CC+WC) were tested in laboratory columns for 5 mo at a 12‐h hydraulic residence time in separate experiments at 15.5 and 1.5°C. Nitrate‐N removal rates ranged from 35 to 1.4 at 15.5°C and from 7.4 to 1.6 g N m −3 d −1 at 1.5°C, respectively; NRRs were ranked CC > CC+WC > BS = CS > WC and CC ≥ CC+WC = CS ≥ BS > WC for 15.5 and 1.5°C, respectively. Although NRRs for CC were increased relative to WC, CC released greater amounts of carbon. Greater abundance of nitrous oxide (N 2 O) reductase gene ( nosZ ) was supported by crop residues than WC at 15.5°C, and CS and BS supported greater abundance than WC at 1.5°C. Production of N 2 O relative to nitrate removal ( r N 2 O) was consistently greater at 1.5°C (7.5% of nitrate removed) than at 15.5°C (1.9%). The r N 2 O was lowest in CC (1.1%) and CC‐WC (0.9%) and greatest in WC (9.7%). Using a compartment of agricultural residue media in series before wood chips has the potential to improve denitrifying bioreactor nitrate removal rates, but field‐scale verification is needed. Core Ideas A compartment of corn cobs before wood chips increases N removal and reduces C loss. Microbial denitrifier populations for crop residues were higher than for wood chips. Denitrification was limited by C availability at 1.5°C. Nitrate‐N removal does not necessarily correlate to N 2 O production. N 2 O production per nitrate‐N removed was nearly 4× at 1.5 compared with 15.5°C.