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Pyrene Mineralization by Mycobacterium sp. Strain KMS in a Barley Rhizosphere
Author(s) -
Child R.,
Miller C. D.,
Liang Y.,
Sims R. C.,
Anderson A. J.
Publication year - 2007
Publication title -
journal of environmental quality
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.888
H-Index - 171
eISSN - 1537-2537
pISSN - 0047-2425
DOI - 10.2134/jeq2007.0008
Subject(s) - microcosm , mineralization (soil science) , pyrene , rhizosphere , environmental chemistry , bioaugmentation , chemistry , polycyclic aromatic hydrocarbon , mycobacterium , incubation , bacteria , microbiology and biotechnology , food science , bioremediation , biology , biochemistry , organic chemistry , nitrogen , genetics
To determine whether the soil Mycobacterium isolate KMS would mineralize pyrene under rhizosphere conditions, a microcosm system was established to collect radioactive carbon dioxide released from the labeled polycyclic aromatic hydrocarbon. Microcosms were designed as sealed, flow‐through systems that allowed the growth of plants. Experiments were conducted to evaluate mineralization of 14 C‐labeled pyrene in a sand amended with the polycyclic aromatic hydrocarbons degrading Mycobacterium isolate KMS, barley plants, or barley plants with roots colonized by isolate KMS. Mineralization was quantified by collecting the 14 CO 2 produced from 14 C‐labeled pyrene at intervals during the 10‐d incubation period. Roots and foliar tissues were examined for 14 C incorporation. Mass balances for microcosms were determined through combustion of sand samples and collection and quantification of 14 CO 2 evolved from radiolabeled pyrene. No pyrene mineralization was observed in the sterile control systems. Greater release of 14 CO 2 was observed in the system with barley colonized by KMS than in microcosms containing just the bacterium inoculum or sterile barley plants. These findings suggest that phytostimulation of polycyclic aromatic hydrocarbons mineralization could be applied in remediation schemes.